Anti-SURF6 Antibody Picoband™

Product Name

Anti-SURF6 Antibody Picoband™

View all SURF6 Antibodies

SKU/Catalog Number

A11571-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SURF6 Antibody Picoband™ catalog # A11798-1. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SURF6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11571-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human SURF6 recombinant protein (Position: M1-R216). Human SURF6 shares 62.5% amino acid (aa) sequence identity with mouse SURF6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A11571-1 is reactive to SURF6 in Human

Applications

A11571-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

41.45kDa

Background of SURF6

Surfeit locus protein 6 is a protein that in humans is encoded by the SURF6 gene. This gene encodes a conserved protein that is localized to the nucleolus. The encoded protein may function as a nucleolar-matrix protein with nucleic acid-binding properties. There is a pseudogene for this gene on chromosome Y. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SURF6 using anti-SURF6 antibody (A11571-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SURF6 antigen affinity purified polyclonal antibody (Catalog # A11571-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SURF6 at approximately 45 kDa. The expected band size for SURF6 is at 41 kDa.

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Figure 2. IF analysis of SURF6 using anti-SURF6 antibody (A11571-1) and anti-Beta Tubulin antibody (M01857-3).
SURF6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SURF6 Antibody (A11571-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For SURF6 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ30322; RRP14; SURF-6; surfeit 6; surfeit locus protein 6 SURF6 RRP14 surfeit 6 surfeit locus protein 6

For more info on SURF6, check out the SURF6 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SURF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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